TIMP4 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for TIMP4 ELISA 试剂盒 (ABIN2748684)
抗原
See all TIMP4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 41.8 pg/mL
-
原理
- The Mouse TIMP-4 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure TIMP-4 (TIMP4) levels in mouse cell culture media, plasma, and serum.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair detects mouse TIMP-4. Other species not determined.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
-
-
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA Kit
TIMP4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 4 (TIMP4) ELISA KitTIMP4 适用: 兔 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
-
-
应用备注
- Suggested Plasma Dilution: 2 fold
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
-
实验流程
-
Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
-
别名
- TIMP-4
-
背景
-
Gene Symbols: TIMP4
Protein Name (Synonyms): Metalloproteinase inhibitor 4 (Tissue inhibitor of metalloproteinases 4) (TIMP-4)
-
基因ID
- 110595
-
UniProt
- Q9JHB3
抗原 See all TIMP4 ELISA试剂盒
-
