RPS6 ELISA 试剂盒
This 人 和 小鼠 RPS6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 和 小鼠 RPS6.
Quick Overview for RPS6 ELISA 试剂盒 (ABIN2748617)
抗原
See all RPS6 ELISA试剂盒
RPS6
(Ribosomal Protein S6 (RPS6))
抗原表位
pSer235, pSer236
适用
人, 小鼠
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Culture Cells
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原理
- Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibodies provided in this kit recognizes human/mouse/rat RPS6 phosphorylated at Serine-235 / Serine-236 and total RPS6 for comparison.
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产品特性
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- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
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组件
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- uncoated 96-well Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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样本量
- 100 μL
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板类型
- Uncoated
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实验流程
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- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacture's instructions.
- Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
- Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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有效期
- 6 months
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- RPS6 (Ribosomal Protein S6 (RPS6))
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别名
- RPS6
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背景
- Disease resistance protein RPS6, Resistance to Pseudomonas syringae 6 (RPS6)
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基因ID
- 6194
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UniProt
- P62753
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途径
- Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原 See all RPS6 ELISA试剂盒
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