RPS6 ELISA 试剂盒
This 人, 小鼠 和 大鼠 RPS6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 RPS6.
Quick Overview for RPS6 ELISA 试剂盒 (ABIN2748616)
抗原
See all RPS6 ELISA试剂盒
RPS6
(Ribosomal Protein S6 (RPS6))
抗原表位
pSer235, pSer236, total
适用
人, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Lysate, Tissue Lysate
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原理
- Human/Mouse/Rat Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit. This assay semi-quantitatively measures phophorylated RPS6 (Ser235/S236) and Total RPS6 in lysate samples.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-RPS6 (pSer235/Ser236) and total RPS6.
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产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. -
限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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有效期
- 6 months
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- RPS6 (Ribosomal Protein S6 (RPS6))
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别名
- RPS6
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背景
- Ribosomal protein S6 phosphorylated at S235/S236 and total RPS6
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基因ID
- 6194
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UniProt
- P62753
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途径
- Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原 See all RPS6 ELISA试剂盒
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