P-Selectin ELISA 试剂盒
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- 抗原 See all P-Selectin (SELP) ELISA试剂盒
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 40-10000 pg/mL
- 最低检测浓度
- 40 pg/mL
- 应用范围
- ELISA
- 原理
- Rat P-Selectin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects rat P-Selectin and mouse P-Selectin. Other species not determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA Kit
SELP 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 小鼠 AA 42-709 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (citrate), Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 AA 42-771 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples200 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all P-Selectin (SELP) ELISA试剂盒
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- 别名
- P-Selectin (SELP 产品)
- 别名
- PSELECT ELISA Kit, CD62 ELISA Kit, CD62P ELISA Kit, GMP140 ELISA Kit, GRMP ELISA Kit, LECAM3 ELISA Kit, PADGEM ELISA Kit, PSEL ELISA Kit, GMP-140 ELISA Kit, Grmp ELISA Kit, selectin P ELISA Kit, selectin, platelet ELISA Kit, Selp ELISA Kit, SELP ELISA Kit
- 背景
- P-Selectin (SELP)
- 基因ID
- 25651
- UniProt
- P98106
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