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CCL4 ELISA 试剂盒

CCL4 适用: 猕猴 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN2748349
发货至: 中国
  • 抗原 See all CCL4 ELISA试剂盒
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    适用
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    猕猴
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Rhesus Macaque MIP-1 beta (CCL4) ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Rhesus Macaque MIP-1 beta (CCL4)
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CCL4 ELISA试剂盒
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    别名
    MIP-1 beta (CCL4 产品)
    别名
    ACT2 ELISA Kit, AT744.1 ELISA Kit, G-26 ELISA Kit, HC21 ELISA Kit, LAG-1 ELISA Kit, LAG1 ELISA Kit, MIP-1-beta ELISA Kit, MIP1B ELISA Kit, MIP1B1 ELISA Kit, SCYA2 ELISA Kit, SCYA4 ELISA Kit, CCL4 ELISA Kit, K203 ELISA Kit, Mip1-b ELISA Kit, Scya4 ELISA Kit, MIP-1beta ELISA Kit, MIP-1BETA ELISA Kit, MIP1b ELISA Kit, SCY4A ELISA Kit, Act-2 ELISA Kit, MIP-1B ELISA Kit, Mip1b ELISA Kit, ACT-2 ELISA Kit, AT744.2 ELISA Kit, CCL4L ELISA Kit, SCYA4L ELISA Kit, SCYA4L1 ELISA Kit, SCYA4L2 ELISA Kit, C-C motif chemokine ligand 4 ELISA Kit, chemokine (C-C motif) ligand 4 ELISA Kit, chemokine (C-C motif) ligand 4-like 1 ELISA Kit, C-C motif chemokine ligand 4 like 1 ELISA Kit, CCL4 ELISA Kit, Ccl4 ELISA Kit, CCL4L1 ELISA Kit
    背景
    MIP-1 beta (CCL4)
    基因ID
    574217
    UniProt
    Q8HYQ2
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