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LGALS1/Galectin 1 ELISA 试剂盒

人和大鼠 LGALS1/Galectin 1 ELISA Kit, Colorimetric is an assay for quantification of 人和大鼠 LGALS1/Galectin 1.

产品编号 ABIN2748113

发货至: 中国

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中国
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Quick Overview for LGALS1/Galectin 1 ELISA 试剂盒 (ABIN2748113)

抗原

See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒

LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

适用

人, 大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.15 ng/mL - 25 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product LGALS1 ELISA Kit

最低检测浓度

0.15 ng/mL

原理

Rat Galectin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB (TNFRSF9/CD137), Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK (CCL27), Decorin, Eotaxin-1 (CCL11), EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-3, Gas 1, GFR alpha-1 (GDNF R), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A (CD321/F11R), MIP-1 alpha (CCL3), Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES (CCL5), SCF, TIM1 (KIM-1), TIMP-2, TREM-1, TWEAK R (TNFRSF12).

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
,
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
LGALS1 适用: Cow Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Lectin, Galactoside-Binding, Soluble, 1 (LGALS1) ELISA Kit
Small Sample LGALS1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

应用备注

Suggested Plasma Dilution: 2 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
实验流程

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

有效期

6 months
抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒

LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

别名

Galectin-1

背景

Gene Symbols: LGALS1
Protein Name (Synonyms): Galectin-1 (Gal-1) (14 kDa lectin) (Beta-galactoside-binding lectin L-14-I) (Galaptin) (Lactose-binding lectin 1) (Lectin galactoside-binding soluble 1) (RL 14.5) (S-Lac lectin 1)

基因ID

56646

UniProt

P11762

途径

Carbohydrate Homeostasis