Decorin ELISA 试剂盒
Quick Overview for Decorin ELISA 试剂盒 (ABIN2748038)
抗原
See all Decorin (DCN) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 20 pg/mL
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原理
- Rat Decorin ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, CD80, CD48, CTACK, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1, TWEAK R.
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灵敏度
- 20 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Decorin (DCN) ELISA Kit
DCN 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Decorin (DCN) ELISA KitDCN 适用: 人 Colorimetric Sandwich ELISA 1.5-700 pg/mL Cell Culture Supernatant, Plasma, Serum
Decorin (DCN) ELISA KitDCN 适用: 小鼠 AA 31-354 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Decorin (DCN) ELISA KitDCN 适用: 人 AA 31-359 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Decorin (DCN) ELISA KitDCN 适用: 小鼠 Colorimetric Sandwich ELISA 5-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Decorin (DCN) ELISA KitDCN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Recommended Dilution for serum and plasma samples5 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- Decorin (DCN)
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别名
- Decorin
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背景
- Decorin (Bone proteoglycan II) (Dermatan sulfate proteoglycan-II) (DSPG) (PG-S2) (PG40)
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基因ID
- 29139
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UniProt
- Q01129
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途径
- Glycosaminoglycan Metabolic Process
抗原 See all Decorin (DCN) ELISA试剂盒
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