电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

BCAM ELISA 试剂盒

This anti-BCAM antibody (ABIN2747976) is a antibody detecting BCAM in ELISA. Suitable for Rhesus Monkey.
产品编号 ABIN2747976
发货至: 中国

Quick Overview for BCAM ELISA 试剂盒 (ABIN2747976)

抗原

See all BCAM ELISA试剂盒
BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

适用

  • 4
  • 2
  • 1
  • 1
猕猴

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • 原理

    Custom Rhesus Macaque Basal Cell Adhesion Molecule (BCAM) ELISA Kit.

    Analytical Method

    Quantitative

    特异性

    The antibody pair provided in this kit recognizes Rhesus Macaque Basal Cell Adhesion Molecule (BCAM)

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all BCAM ELISA试剂盒

    BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

    别名

    BCAM

    背景

    Basal Cell Adhesion Molecule (BCAM)

    UniProt

    H9Z2N4
You are here:
Chat with us!