Transferrin ELISA 试剂盒
Quick Overview for Transferrin ELISA 试剂盒 (ABIN2703533)
抗原
See all Transferrin (TF) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1 ng/mL
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原理
- Human Transferrin ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human Transferrin. Other species not determined.
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灵敏度
- 1 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Transferrin (TF) ELISA Kit
TF 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin (TF) ELISA KitHigh Sensitivity TF 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin (TF) ELISA KitTF 适用: 小鼠 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin (TF) ELISA KitHigh Sensitivity TF 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transferrin (TF) ELISA KitTF 适用: 犬 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Transferrin (TF) ELISA KitTF 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Transferrin (TF) ELISA KitTF 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Plasma, Serum, Urine
Transferrin (TF) ELISA KitTF 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Transferrin (TF) ELISA KitTF 适用: 小鸡 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transferrin (TF) ELISA KitTF 适用: 人 Colorimetric Sandwich ELISA 9.375 ng/mL - 600 ng/mL Plasma, Serum, Urine
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应用备注
- Recommended Dilution for serum and plasma samples20,000 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1x Assay Diluent D to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL 1x Assay Diluent to prepare a final 20,000-fold diluted sample.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- Transferrin (TF)
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别名
- Transferrin
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背景
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Gene Names: TF PRO1400
Protein names: Serotransferrin (Transferrin) (Beta-1 metal-binding globulin) (Siderophilin)
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基因ID
- 7018
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UniProt
- P02787
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途径
- Transition Metal Ion Homeostasis
抗原 See all Transferrin (TF) ELISA试剂盒
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