Thyroperoxidase ELISA 试剂盒
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- 抗原 See all Thyroperoxidase (TPO) ELISA试剂盒
- Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.3-100 ng/mL
- 最低检测浓度
- 0.3 ng/mL
- 应用范围
- ELISA
- 原理
- Human Thyroid Peroxidase ELISA Kit for cell culture supernatants, EDTA treated plasma, and serum samples. Heparin and citrate are not recommended.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human TPX. Other species not determined.
- 灵敏度
- 0.3 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Thyroid Peroxidase (TPO) ELISA Kit
TPO 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 人 Colorimetric Sandwich ELISA 0.9 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 大鼠 Colorimetric Competition ELISA 2.5-40 ng/mL Plasma, Serum, Tissue Homogenate
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 人 Colorimetric Sandwich ELISA 64-4000 pg/mL Cell Lysate, Plasma, Serum
Thyroid Peroxidase (TPO) ELISA KitTPO 适用: 小鼠 Colorimetric Sandwich ELISA 2.5-40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Thyroperoxidase (TPO) ELISA试剂盒
- Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))
- 别名
- Thyroid Peroxidase (TPO 产品)
- 别名
- MSA ELISA Kit, TDH2A ELISA Kit, TPX ELISA Kit, TPO ELISA Kit, msa ELISA Kit, tpx ELISA Kit, xtpo ELISA Kit, LOC100218312 ELISA Kit, thyroid peroxidase ELISA Kit, TPO ELISA Kit, Tpo ELISA Kit, tpo ELISA Kit, LOC100547103 ELISA Kit
- 背景
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Gene Names: TPO
Protein names: Thyroid peroxidase (TPO) (EC 1.11.1.8)
- 基因ID
- 7173
- UniProt
- P07202
- 途径
- Thyroid Hormone Synthesis
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