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tau ELISA 试剂盒

This anti-tau antibody (ABIN2703476) is a antibody detecting tau in ELISA. Suitable for Human.
产品编号 ABIN2703476
发货至: 中国

Quick Overview for tau ELISA 试剂盒 (ABIN2703476)

抗原

See all tau ELISA试剂盒
tau (tau Protein)

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

100-25.000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum
  • 最低检测浓度

    100 pg/mL

    原理

    Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

    Analytical Method

    Quantitative

    特异性

    This ELISA antibody pair detects human Tau. Other species not determined.

    灵敏度

    100 pg/mL

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注

    Recommended Dilution for serum and plasma samples2 fold

    样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    样品制备

    Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all tau ELISA试剂盒

    tau (tau Protein)

    别名

    tau

    背景

    Gene Names: MAPT MAPTL MTBT1 TAU
    Protein names: Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

    基因ID

    4137

    UniProt

    P10636
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