DLK1 ELISA 试剂盒
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- 抗原 See all DLK1 ELISA试剂盒
- DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.4-100 ng/mL
- 最低检测浓度
- 0.4 ng/mL
- 应用范围
- ELISA
- 原理
- Human Pref-1 (DLK-1/FA1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-12, ADAM-8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, Coagulation Factor XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein-2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, I-COS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin-3, Siglec-10, SLAM, SP-D, Syndecan-4,Testican 2, TIM-3, TLR4, TRAIL, ULBP-1.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling DLK1 ELISA Kit
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- Discover our top product DLK1 ELISA Kit
-
delta-Like 1 Homolog (Drosophila) (DLK1) ELISA Kit
DLK1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 Homolog (Drosophila) (DLK1) ELISA KitDLK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 Homolog (Drosophila) (DLK1) ELISA KitDLK1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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-
: "The adipokine preadipocyte factor-1 is downregulated in preeclampsia and expressed in placenta." in: Cytokine, Vol. 75, Issue 2, pp. 338-43, (2015) (PubMed).
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: "The adipokine preadipocyte factor-1 is downregulated in preeclampsia and expressed in placenta." in: Cytokine, Vol. 75, Issue 2, pp. 338-43, (2015) (PubMed).
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- 抗原 See all DLK1 ELISA试剂盒
- DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))
- 别名
- Pref-1 (DLK1 产品)
- 别名
- DELTA1 ELISA Kit, DL1 ELISA Kit, Delta ELISA Kit, DLK ELISA Kit, DLK-1 ELISA Kit, Delta1 ELISA Kit, FA1 ELISA Kit, PREF1 ELISA Kit, Pref-1 ELISA Kit, ZOG ELISA Kit, pG2 ELISA Kit, AW742678 ELISA Kit, DlkI ELISA Kit, Ly107 ELISA Kit, Peg9 ELISA Kit, SCP1 ELISA Kit, pref-1 ELISA Kit, Zog ELISA Kit, delta like canonical Notch ligand 1 ELISA Kit, delta like non-canonical Notch ligand 1 ELISA Kit, delta-like 1 homolog (Drosophila) ELISA Kit, delta-like 1 (Drosophila) ELISA Kit, DLL1 ELISA Kit, DLK1 ELISA Kit, Dll1 ELISA Kit, Dlk1 ELISA Kit
- 背景
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Gene Names: DLK1 DLK
Protein names: Protein delta homolog 1 (DLK-1) (pG2) [Cleaved into: Fetal antigen 1 (FA1)]
- 基因ID
- 8788
- UniProt
- P80370
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