Osteocalcin ELISA 试剂盒
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- 抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
- Osteocalcin (BGLAP)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.4-100 ng/mL
- 最低检测浓度
- 0.4 ng/mL
- 应用范围
- ELISA
- 原理
- Human Osteocalcin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human Osteocalcin. Other species not determined.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Osteocalcin (BGLAP) ELISA Kit
BGLAP 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鸡 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 豚鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 猴 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples5 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
- Osteocalcin (BGLAP)
- 别名
- Osteocalcin (BGLAP 产品)
- 别名
- BGP ELISA Kit, OC ELISA Kit, OCN ELISA Kit, Bglap1 ELISA Kit, Bglap2 ELISA Kit, OG1 ELISA Kit, mOC-A ELISA Kit, Bgp ELISA Kit, Bgpr ELISA Kit, Bgpra ELISA Kit, bgp ELISA Kit, LOC792433 ELISA Kit, ocn ELISA Kit, GLA ELISA Kit, BGLAP ELISA Kit, PMF1 ELISA Kit, bone gamma-carboxyglutamate protein ELISA Kit, bone gamma carboxyglutamate protein ELISA Kit, bone gamma-carboxyglutamate protein S homeolog ELISA Kit, bone gamma-carboxyglutamate (gla) protein ELISA Kit, osteocalcin ELISA Kit, polyamine-modulated factor 1 ELISA Kit, BGLAP ELISA Kit, Bglap ELISA Kit, bglap.S ELISA Kit, bglap ELISA Kit, ocn ELISA Kit, LOC100529177 ELISA Kit, LOC100146589 ELISA Kit
- 背景
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Gene Names: BGLAP
Protein names: Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
- 基因ID
- 632
- UniProt
- P02818
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