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OLR1 ELISA 试剂盒
OLR1
适用: 人
Colorimetric
Sandwich ELISA
2-500 pg/mL
Cell Culture Supernatant, Plasma, Serum
OLR1ELISA Kit详情
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OLR1
(Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
适用
All reactivities for OLR1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
2-500 pg/mL
最低检测浓度
2 pg/mL
应用范围
ELISA
原理
Human LOX-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair detects human LOX-1. Other species not determined yet.
灵敏度
2 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 - 10 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
OLR1目标详情
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抗原
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OLR1
(Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
别名
LOX-1 (OLR1 产品 )
别名
OLR1 ELISA Kit, LOX1 ELISA Kit, Olr1 ELISA Kit, CLEC8A ELISA Kit, LOXIN ELISA Kit, SCARE1 ELISA Kit, SLOX1 ELISA Kit, LOX-1 ELISA Kit, SR-EI ELISA Kit, Scare1 ELISA Kit, Oldlr1 ELISA Kit, Oldr1 ELISA Kit, PLOX-1 ELISA Kit, oxidized low density lipoprotein receptor 1 ELISA Kit, oxidized low density lipoprotein (lectin-like) receptor 1 ELISA Kit, OLR1 ELISA Kit, Olr1 ELISA Kit
背景
Gene Names: OLR1 CLEC8A LOX1 Protein names: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) (C-type lectin domain family 8 member A) (Lectin-like oxidized LDL receptor 1) (LOX-1) (Lectin-like oxLDL receptor 1) (hLOX-1) (Lectin-type oxidized LDL receptor 1) [Cleaved into: Oxidized low-density lipoprotein receptor 1, soluble form]
基因ID
4973
UniProt
P78380
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