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IL-33 ELISA 试剂盒

The 小鼠 IL-33 ELISA Kit (ABIN2703232) is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-33. This ELISA Kit has been cited in 2 publications.

ELISA

1 image

(2 references)

产品编号 ABIN2703232

发货至: 中国

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Quick Overview for IL-33 ELISA 试剂盒 (ABIN2703232)

抗原

See all IL-33 (IL33) ELISA试剂盒

IL-33 (IL33) (Interleukin 33 (IL33))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

2 pg/mL - 500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product IL33 ELISA Kit

最低检测浓度

2 pg/mL

原理

Mouse IL-33 (IL-1 F11) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method

Quantitative

特异性

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48. CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PIGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
,
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 犬 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 AA 112-270 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Interleukin 33 (IL33) ELISA Kit
IL33 适用: 小鼠 AA 109-266 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

应用备注

Suggested Plasma Dilution: 2 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Zhang, Wang, Li, Wu, Si, Wu: "Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice." in: Medicines (Basel, Switzerland), Vol. 3, Issue 3, (2016) (PubMed).

Raeiszadeh Jahromi, Mahesh, Jayaraj, Madhunapantula, Holla, Vishweswaraiah, Ramachandra: "Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case-control study." in: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 51, Issue 10, pp. 1004-13, (2014) (PubMed).
抗原 See all IL-33 (IL33) ELISA试剂盒

IL-33 (IL33) (Interleukin 33 (IL33))

别名

IL-33

背景

Gene Symbols: IL33
Protein Name (Synonyms): Interleukin-33 (IL-33) [Cleaved into: Interleukin-33(102-266) Interleukin-33(109-266)

基因ID

77125

UniProt

Q8BVZ5

途径

Production of Molecular Mediator of Immune Response