IL-33 ELISA 试剂盒
(2 references)Quick Overview for IL-33 ELISA 试剂盒 (ABIN2703232)
抗原
See all IL-33 (IL33) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 2 pg/mL
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原理
- Mouse IL-33 (IL-1 F11) ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48. CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PIGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon
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灵敏度
- 2 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 33 (IL33) ELISA Kit
IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Urine
Interleukin 33 (IL33) ELISA KitIL33 适用: 犬 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 AA 109-266 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 AA 112-270 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 33 (IL33) ELISA KitIL33 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Recommended Dilution for serum and plasma samples2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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: "Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice." in: Medicines (Basel, Switzerland), Vol. 3, Issue 3, (2016) (PubMed).
: "Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case-control study." in: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 51, Issue 10, pp. 1004-13, (2014) (PubMed).
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: "Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice." in: Medicines (Basel, Switzerland), Vol. 3, Issue 3, (2016) (PubMed).
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- IL-33 (IL33) (Interleukin 33 (IL33))
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别名
- IL-33
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背景
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Gene Names: Il33
Protein names: Interleukin-33 (IL-33) [Cleaved into: Interleukin-33(102-266), Interleukin-33(109-266)]
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基因ID
- 77125
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UniProt
- Q8BVZ5
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途径
- Production of Molecular Mediator of Immune Response
抗原 See all IL-33 (IL33) ELISA试剂盒
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