电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

IGF2R ELISA 试剂盒

This anti-IGF2R antibody (ABIN2703119) is a antibody detecting IGF2R in ELISA. Suitable for Human.
产品编号 ABIN2703119
发货至: 中国

Quick Overview for IGF2R ELISA 试剂盒 (ABIN2703119)

抗原

See all IGF2R ELISA试剂盒
IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

适用

  • 5
  • 1
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.27-200 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum
  • 最低检测浓度

    0.27 ng/mL

    原理

    Human IGF-2 R ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

    Analytical Method

    Quantitative

    特异性

    The antibody pair provided in this kit recognizes Human Insulin-Like Growth Gactor type II Receptor.

    灵敏度

    0.27 ng/mL

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注

    Recommended Dilution for serum and plasma samples2 fold

    样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all IGF2R ELISA试剂盒

    IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

    别名

    IGF2R

    背景

    Gene Names: IGF2R MPRI
    Protein names: Cation-independent mannose-6-phosphate receptor (CI Man-6-P receptor) (CI-MPR) (M6PR) (300 kDa mannose 6-phosphate receptor) (MPR 300) (Insulin-like growth factor 2 receptor) (Insulin-like growth factor II receptor) (IGF-II receptor) (M6P/IGF2 receptor) (M6P/IGF2R) (CD antigen CD222)

    基因ID

    3482

    UniProt

    P11717
You are here:
Chat with us!