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CFHR5 ELISA 试剂盒

CFHR5 适用: 人 Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2702908
发货至: 中国
  • 抗原 See all CFHR5 ELISA试剂盒
    CFHR5 (Complement Factor H-Related 5 (CFHR5))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.41-100 ng/mL
    最低检测浓度
    0.41 ng/mL
    应用范围
    ELISA
    原理
    Human Complement Factor H-related 5 (CFHR5) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Human Complement Factor H-related 5 (CFHR5)
    灵敏度
    0.41 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples400 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    样品制备

    For example, add 2 μL of serum or plasma into a tube with 798 μL 1X Assay Diluent to prepare a 400-fold diluted sample. Mix well.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CFHR5 ELISA试剂盒
    CFHR5 (Complement Factor H-Related 5 (CFHR5))
    别名
    CFHR5 (CFHR5 产品)
    别名
    CFHL5 ELISA Kit, CFHR5D ELISA Kit, FHR-5 ELISA Kit, FHR5 ELISA Kit, complement factor H related 5 ELISA Kit, CFHR5 ELISA Kit
    背景
    Gene Names: CFHR5 CFHL5 FHR5
    Protein names: Complement factor H-related protein 5 (FHR-5)
    基因ID
    81494
    UniProt
    Q9BXR6
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