LY9 ELISA 试剂盒
This 人 LY9 ELISA Kit specifically detects LY9
in Cell Culture Supernatant, Plasma 和 Serum.
1 image
产品编号 ABIN2702892
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for LY9 ELISA 试剂盒 (ABIN2702892)
抗原
See all LY9 (CD229) ELISA试剂盒
LY9 (CD229)
(Lymphocyte Antigen 9 (CD229))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
80-20.000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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最低检测浓度
- 80 pg/mL
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原理
- Human CD229 (SLAMF3) ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1 .
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灵敏度
- 80 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Lymphocyte Antigen 9 (CD229) ELISA Kit
CD229 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples3 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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别名
- CD229
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背景
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Gene Names: LY9 CDABP0070
Protein names: T-lymphocyte surface antigen Ly-9 (Cell surface molecule Ly-9) (Lymphocyte antigen 9) (SLAM family member 3) (SLAMF3) (Signaling lymphocytic activation molecule 3) (CD antigen CD229)
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基因ID
- 4063
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UniProt
- Q9HBG7
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途径
- BCR Signaling
抗原 See all LY9 (CD229) ELISA试剂盒
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