Apolipoprotein C-II ELISA 试剂盒
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Quick Overview for Apolipoprotein C-II ELISA 试剂盒 (ABIN2702831)
抗原
See all Apolipoprotein C-II (APOC2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.5 ng/mL
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原理
- Human ApoC2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
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灵敏度
- 1.5 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Apolipoprotein C-II (APOC2) ELISA Kit
APOC2 适用: 小鼠 Colorimetric Competition ELISA 18.8 ng/mL - 1200 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 人 Colorimetric Competition ELISA 31.2 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-II (APOC2) ELISA KitWide Range APOC2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 小鼠 Colorimetric Sandwich ELISA 18.8-1200 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-II (APOC2) ELISA KitAPOC2 适用: 人 Colorimetric Sandwich ELISA 0.063-4 μg/mL Cell Culture Cells, Plasma, Serum
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应用备注
- Recommended Dilution for serum and plasma samples20,000 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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For example, add 2 μL of serum/plasma into a tube with 198.0 μL Assay Diluent C to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL Assay Diluent C to prepare a final 20,000 fold diluted sample.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- Apolipoprotein C-II (APOC2)
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别名
- ApoC2
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背景
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Gene Names: APOC2 APC2
Protein names: Apolipoprotein C-II (Apo-CII) (ApoC-II) (Apolipoprotein C2) [Cleaved into: Proapolipoprotein C-II (ProapoC-II)]
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基因ID
- 344
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UniProt
- P02655
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途径
- Lipid Metabolism
抗原 See all Apolipoprotein C-II (APOC2) ELISA试剂盒
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