APOA2 ELISA 试剂盒
-
- 抗原 See all APOA2 ELISA试剂盒
- APOA2 (Apolipoprotein A-II (APOA2))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.025-20 ng/mL
- 最低检测浓度
- 0.025 ng/mL
- 应用范围
- ELISA
- 原理
- Human ApoA2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human ApoA2. Other species not determined.
- 灵敏度
- 0.025 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling APOA2 ELISA Kit
- Top Product
- Discover our top product APOA2 ELISA Kit
-
Apolipoprotein A-II (APOA2) ELISA Kit
APOA2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 人 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 人 Colorimetric Sandwich ELISA 21.875 ng/mL - 1400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Apolipoprotein A-II (APOA2) ELISA KitAPOA2 适用: Pig Colorimetric Sandwich ELISA 4.69 μg/mL - 300 μg/mL Plasma, Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples100,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
-
For example, add 2 μL of serum or plasma into a tube with 398 μL 1X Assay Diluent to prepare a 200-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 200-fold diluted sample into a tube with 998 μL 1X Assay Diluent to prepare a final 100,000-fold diluted sample.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all APOA2 ELISA试剂盒
- APOA2 (Apolipoprotein A-II (APOA2))
- 别名
- ApoA2 (APOA2 产品)
- 别名
- Apo-AII ELISA Kit, ApoA-II ELISA Kit, apoAII ELISA Kit, Alp-2 ELISA Kit, ApoAII ELISA Kit, Apoa-2 ELISA Kit, Hdl-1 ELISA Kit, APOAII ELISA Kit, apoa2 ELISA Kit, apoA-II ELISA Kit, cb1032 ELISA Kit, wu:fb57h11 ELISA Kit, zgc:193613 ELISA Kit, apolipoprotein A2 ELISA Kit, apolipoprotein A-II ELISA Kit, APOA2 ELISA Kit, Apoa2 ELISA Kit, apoa2 ELISA Kit
- 背景
-
Gene Names: APOA2
Protein names: Apolipoprotein A-II (Apo-AII) (ApoA-II) (Apolipoprotein A2) [Cleaved into: Proapolipoprotein A-II (ProapoA-II), Truncated apolipoprotein A-II (Apolipoprotein A-II(1-76))]
- 基因ID
- 336
- UniProt
- P02652
- 途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism
-
