APOA1 ELISA 试剂盒
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- 抗原 See all APOA1 ELISA试剂盒
- APOA1 (Apolipoprotein A-I (APOA1))
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适用
- 人, 猕猴
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.08-20 ng/mL
- 最低检测浓度
- 0.08 ng/mL
- 应用范围
- ELISA
- 原理
- Human ApoA ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.
- 灵敏度
- 0.08 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling APOA ELISA Kit
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- Discover our top product APOA1 ELISA Kit
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Apolipoprotein A-I (APOA1) ELISA Kit
APOA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 500 ng/mL Plasma, Serum, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 犬 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: Pig Colorimetric Sandwich ELISA 1.563 μg/mL - 90 μg/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 兔 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples1,000,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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For example, add 1 μL of serum or plasma into a tube with 999 μL 1X Assay Diluent to prepare a 1,000-fold diluted sample. Mix thoroughly and then pipette 1 μL of prepared 1,000-fold diluted sample into a tube with 999 μL 1X Assay Diluent to prepare a final 1,000,000-fold diluted sample.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all APOA1 ELISA试剂盒
- APOA1 (Apolipoprotein A-I (APOA1))
- 别名
- APOA1 (APOA1 产品)
- 别名
- Alp-1 ELISA Kit, Apoa-1 ELISA Kit, Brp-14 ELISA Kit, Ltw-1 ELISA Kit, Lvtw-1 ELISA Kit, Sep-1 ELISA Kit, Sep-2 ELISA Kit, Sep2 ELISA Kit, apoA-I ELISA Kit, apoa ELISA Kit, apoa1 ELISA Kit, cb49 ELISA Kit, wu:fb33f01 ELISA Kit, zgc:103718 ELISA Kit, MGC64335 ELISA Kit, MGC89745 ELISA Kit, Apo-AI ELISA Kit, ApoA-I ELISA Kit, APOA1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, ApoAI ELISA Kit, apolipoprotein A1 ELISA Kit, apolipoprotein A-I ELISA Kit, apolipoprotein A-Ia ELISA Kit, apolipoprotein A-I S homeolog ELISA Kit, apolipoprotein A4 ELISA Kit, APOA1 ELISA Kit, Apoa1 ELISA Kit, apoa1a ELISA Kit, apoa1.S ELISA Kit, apoa1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, apoa4 ELISA Kit
- 背景
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Gene Names: APOA1
Protein names: Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I), Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]
- 基因ID
- 335
- UniProt
- P02647
- 途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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