APOA1 ELISA 试剂盒
Quick Overview for APOA1 ELISA 试剂盒 (ABIN2702827)
抗原
See all APOA1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.08 ng/mL
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原理
- Human ApoA ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.
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灵敏度
- 0.08 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Apolipoprotein A-I (APOA1) ELISA Kit
APOA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 500 ng/mL Plasma, Serum, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 兔 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: Pig Colorimetric Sandwich ELISA 1.563 μg/mL - 90 μg/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 犬 Colorimetric Competition ELISA 28.12 ng/mL - 1800 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples1,000,000 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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For example, add 1 μL of serum or plasma into a tube with 999 μL 1X Assay Diluent to prepare a 1,000-fold diluted sample. Mix thoroughly and then pipette 1 μL of prepared 1,000-fold diluted sample into a tube with 999 μL 1X Assay Diluent to prepare a final 1,000,000-fold diluted sample.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- APOA1 (Apolipoprotein A-I (APOA1))
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别名
- APOA1
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背景
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Gene Names: APOA1
Protein names: Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I), Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]
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基因ID
- 335
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UniProt
- P02647
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途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
抗原 See all APOA1 ELISA试剂盒
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