IL23 ELISA 试剂盒
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北京 101111
Quick Overview for IL23 ELISA 试剂盒 (ABIN2681248)
抗原
See all IL23 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.63 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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特异性
- IL-23 ELISA Kit, Mouse
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Interleukin 23 (IL23) ELISA Kit
IL23 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: 人 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 23 (IL23) ELISA KitIL23 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: 绵羊 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: 人 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23 (IL23) ELISA KitIL23 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- IL23 (Interleukin 23 (IL23))
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别名
- IL-23
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背景
- IL-23 protein is a heterodimer, sharing a p40 subunit with IL-12, but having a distinct p19 subunit, which is IL-23 specific. Prior to the discovery of IL-23, IL-12 had been proposed to represent a key mediator of inflammation in mouse models of inflammation.However, many studies aimed at assessing the role of IL-12 had blocked the activity of IL-12p40, and were therefore not as specific as thought. Studies which blocked the function of IL-12p35 did not produce the same results as those targeting IL-12p40 as would have been expected if both subunits formed part of IL-12 only. IL-23 was shown to be involved in inflammation of the nervous system and autoimmune diseases.IL-23 was shown to facilitate development of inflammation in numerous other models of immune pathology where IL-12 had previously been implicated including models of arthritis, intestinal inflammation, and psoriasis.
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基因ID
- 16160
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UniProt
- P43432
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