MMP7 ELISA 试剂盒
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Quick Overview for MMP7 ELISA 试剂盒 (ABIN2681244)
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See all MMP7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4.9 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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特异性
- Human MMP-7 ELISA Kit
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
MMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 AA 18-267 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 山羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 马 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
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别名
- MMP7
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背景
- Matrix metalloproteinase-7 (MMP-7) also known as matrilysin, pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme that in humans is encoded by the MMP7gene. MMP-7 are commonly expressed in epithelial cells including ductal epithelium of exocrine glands in skin, salivary glands, pancreas, glandular epithelium of intestine and reproductive organ, liver, and breast. In addition, MMP7 is highly expressed in the luminal surface of dysplastic glands in human colorectal cancers. The expression of MMP7 is regulated by the Wnt/β catenin signaling pathway, and mediated by transformation growth factor β (TGF-β). The primary role of cleaved/activated MMP7 is to break down extracellular matrix by degrading macromolecules including casein, type I, II, IV, and V gelatins, fibronectin, and proteoglycan. The activated MMP-7 can cleave the propeptides of proMMP-2 and proMMP-9 to facilitate tumor invasion.
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NCBI登录号
- NM_002423
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UniProt
- P09237
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途径
- Production of Molecular Mediator of Immune Response
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