Quick Overview for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213)
抗原
See all Annexin V (ANXA5) 试剂盒适用
应用范围
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标记
- FITC
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产品特性
- FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
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组件
- FITC Annexin V Apoptosis Detection Kit with PI
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Annexin A5 (ANXA5) ELISA Kit
ANXA5 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 猴 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 小鸡 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 马 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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注意事项
- Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store between 2°C and 8°C.
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- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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别名
- Annexin V
抗原
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