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TBARS Cuvette Assay

BCA Plasma, Serum, Urine
产品编号 ABIN2648830
发货至: 中国
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Quick Overview for TBARS Cuvette Assay (ABIN2648830)

抗原

TBARS

应用范围

Biochemical Assay (BCA)

样品类型

Serum, Plasma, Urine
  • 原理

    2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.

    灵敏度

    0.5 μM

    产品特性

    2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
  • 样本量

    400 μL

    实验时间

    < 0.5 h

    实验流程

    This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原

    TBARS
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