Osteocalcin ELISA 试剂盒
Quick Overview for Osteocalcin ELISA 试剂盒 (ABIN2648801)
抗原
See all Osteocalcin (BGLAP) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- .31 ng/ml
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产品特性
- Human Osteocalcin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of both human osteocalcin (1-49) and osteocalcin (1-43) (also referred as N-terminal & mid-regional osteocalcin) levels in test samples). Human Osteocalcin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Osteocalcin (BGLAP) ELISA Kit
BGLAP 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鸡 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 猴 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitHigh Sensitivity BGLAP 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 25 μL
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实验时间
- 1.5 h
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实验流程
- Human Osteocalcin ELISA Assay Kit is designed, developed and produced for the quantitative measurement of of human osteocalcin (1-49) and (1-43) in serum or plasma sample. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human osteocalcin. Assay standards, controls and samples are added directly to wells of a microtiter plate that is coated with streptavidin. Subsequently, a mixture of biotinylated human osteocalcin N-terminal region specific polyclonal antibody and a peroxidase labeled human osteocalcin 20 - 43 region specific monoclonal antibody is added to each well. After the first incubation period, a "sandwich" of "biotinylated antibody - human osteocalcin - HRP-monoclonal antibody" is formed and this immunocomplex is also captured to the wall of microtiter plate via a streptavidin-biotin affinity binding. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. A substrate solution in a timed reaction is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human osteocalcin in a test sample. A standard curve is generated by plotting the absorbance versus the respective human osteocalcin concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of human osteocalcin in test samples is determined directly from this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Osteocalcin (BGLAP)
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别名
- Osteocalcin
抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
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