ENO2/NSE ELISA 试剂盒
Quick Overview for ENO2/NSE ELISA 试剂盒 (ABIN2648800)
抗原
See all ENO2/NSE (ENO2) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 1.2 ng/ml
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产品特性
- Human Neuron Specific Enolase (NSE) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human neuron specific enolase (NSE) levels in serum. Human Neuron Specific (NSE) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Enolase 2 (Gamma, Neuronal) (ENO2) ELISA Kit
ENO2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 人 Colorimetric Sandwich ELISA 1.2-178 ng/mL Serum
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 兔 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: 绵羊 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Enolase 2 (Gamma, Neuronal) (ENO2) ELISA KitENO2 适用: Pig Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 10 μL
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实验时间
- 1 h
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实验流程
- Human Neuron Specific Enolase (NSE) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human NSE in serum sample. The assay utilizes the two-site "sandwich" technique with two selected monoclonal antibodies that bind to different epitopes of the γ-subunit of the enzyme. Assay standards, controls and patient samples are added directly to wells of microplate that is coated with a streptavidin. Subsequently, a mixture of a biotinylated NSE specific monoclonal antibody and a horseradish peroxidase (HRP) labeled NSE specific monoclonal antibody is added to each microtiter well. After the first incubation a "sandwich" immunocomplex of "streptavidin-biotin-monoclonal antibody - human NSE - monoclonal antibody-HRP" is formed. The unbound monoclonal antibodies are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the NSE on the wall of the microtiter well is directly proportional to the amount of NSE in the sample. A standard curve is generated by plotting the absorbance versus the respective human NSE concentration for each standard on point-to-point, cubical scales or 4 parameter curve fit. The concentration of human NSE in test samples is determined directly from this standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- Neuron Enolase (NSE)
抗原 See all ENO2/NSE (ENO2) ELISA试剂盒
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