Normetanephrine & Metanephrine ELISA 试剂盒
The 化学剂 Normetanephrine & Metanephrine ELISA Kit (ABIN2648796) is a Colorimetric ELISA Kit designed to quantify 化学剂 Normetanephrine & Metanephrine.
产品编号 ABIN2648796
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Normetanephrine & Metanephrine ELISA 试剂盒 (ABIN2648796)
抗原
Normetanephrine & Metanephrine
适用
化学剂
检测方法
Colorimetric
应用范围
ELISA
样品类型
Plasma (EDTA)
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原理
- The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma.
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Analytical Method
- Quantitative
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灵敏度
- Metanephrine: 10 pg/ml, Normetanephrine: 15 pg/ml
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产品特性
- Normetanephrine and metanephrine are physiologically formed from the catechol-amines noradrenaline and adrenaline by the enzyme catechol-O-methyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in pheochromocytoma, ganglioneuroma and other neurogenic tumors. The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma. Normetanephrine and metanephrine are quantitatively acylated to their N-acyl-derivates.
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 400 μL
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实验流程
- The competitive Normetanephrine & Metanephrine (Plasma) ELISA Kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Normetanephrine & Metanephrine
抗原
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