GM-CSF ELISA 试剂盒
Quick Overview for GM-CSF ELISA 试剂盒 (ABIN2648793)
抗原
See all GM-CSF (CSF2) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 7 pg/ml
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产品特性
- Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA Kit
CSF2 适用: 小鼠 Colorimetric Sandwich ELISA 1.37 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 兔 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 人 AA 18-144 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 小鼠 AA 18-141 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 AA 1-127 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 0.02-1.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GM-CSF
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原 See all GM-CSF (CSF2) ELISA试剂盒
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