MMP7 ELISA 试剂盒
Quick Overview for MMP7 ELISA 试剂盒 (ABIN2648787)
抗原
See all MMP7 ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 1 ng/ml
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产品特性
- MMP-7 proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The MMP-7 enzyme degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
MMP7 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 马 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 山羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 AA 18-267 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 total Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
SensoLyte® 520 MMP-7 Assay KitFRET Fluorometric
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA KitMMP7 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- MMP-7 ELISA test kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact MMP-7 Molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a biotin conjugated anti-MMP-7 monoclonal antibody. After a second wash Streptavidin conjugated to HRP is added as a reporting agent. Excess streptavidin-HRP is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if MMP-7 is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of MMP-7 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
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别名
- MMP-7
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途径
- Production of Molecular Mediator of Immune Response
抗原 See all MMP7 ELISA试剂盒
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