Malondialdehyde (MDA) HPLC Assay
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- 抗原 See all Malondialdehyde (MDA) 试剂盒
- Malondialdehyde (MDA)
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适用
- 化学剂
- 应用范围
- High Pressure Liquid Chromatography (HPLC)
- 原理
- In a healthy body, oxidative and reductive processes are in a balance. Free radicals (reactive oxygen species) are eliminated by antioxidants. In case of a lack of antioxidants free radicals react with cell structures. A reaction of free radicals with unsaturated fatty acids leads to lipid peroxidation products. A reaction of free radicals with poly-unsaturated fatty acids generates malondialdehyde and/or 4-hydroxynonenal. These secondary lipid peroxidation products might react with other molecules in the cell. Modifications of the DNA-based adenine or guanine result in incorrect transcripts. A reaction of free radicals with proteins leads to an alteration or loss of function. The creation of neoantigens is possible. Neoantigens are recognized by the immune system, thus resulting in autoimmune diseases. The participation of lipid peroxidation products have been discussed in several diseases such as atherosclerosis, tumor genesis, rheumatism and reperfusion injury after transplantation.
- 样品类型
- Serum, Plasma
- 产品特性
- In a healthy body, oxidative and reductive processes are in a balance. Free radicals (reactive oxygen species) are eliminated by antioxidants. In case of a lack of antioxidants free radicals react with cell structures. A reaction of free radicals with unsaturated fatty acids leads to lipid peroxidation products. A reaction of free radicals with poly-unsaturated fatty acids generates malondialdehyde and/or 4-hydroxynonenal. These secondary lipid peroxidation products might react with other molecules in the cell. Modifications of the DNA-based adenine or guanine result in incorrect transcripts. A reaction of free radicals with proteins leads to an alteration or loss of function. The creation of neoantigens is possible. Neoantigens are recognized by the immune system, thus resulting in autoimmune diseases. The participation of lipid peroxidation products have been discussed in several diseases such as atherosclerosis, tumor genesis, rheumatism and reperfusion injury after transplantation.
- Top Product
- Discover our top product MDA ELISA Kit
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Malondialdehyde (MDA) ELISA Kit
MDA 适用: Various Species Colorimetric Competition ELISA 24.69 ng/mL - 2000 ng/mL Plasma, Serum
Malondialdehyde (MDA) ELISA KitMDA 适用: 化学剂 Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum
Malondialdehyde (MDA) ELISA KitMDA 适用: Various Species Colorimetric Competition ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Malondialdehyde (MDA) ELISA KitHigh Sensitivity MDA 适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum
Malondialdehyde (MDA) ELISA KitMDA 适用: Various Species Colorimetric Sandwich ELISA 0.312-20 nM/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Malondialdehyde (MDA) ELISA KitMDA 适用: 小鼠 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Malondialdehyde (MDA) ELISA KitMDA 适用: 犬 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 20 μL
- 实验时间
- 1 h
- 实验流程
- For the determination of malondialdehyde a derivatisation step, in which protein bound malondialdehyde is hydrolyzed and converted into a fluorescent probe (60 min at 95 °C) is performed. The fluorescent probe is then cooled (2-8 °C), centrifuged, mixed with a reaction solution and injected into the HPLC system. The isocratic separation via HPLC at 30 °C, using a "reversed phase" column, lasts 4 minutes for one sample. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered calibrator, the concentration is calculated via integration of the peak heights.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Malondialdehyde (MDA)
- Abstract
- MDA 产品
- 物质类
- Chemical
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