ST14 ELISA 试剂盒
This 人 ST14 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ST14.
Quick Overview for ST14 ELISA 试剂盒 (ABIN2648780)
抗原
See all ST14 ELISA试剂盒
ST14
(Suppression of Tumorigenicity 14 (ST14))
适用
人
检测方法
Colorimetric
应用范围
ELISA
样品类型
Serum
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灵敏度
- 2 ng/ml
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产品特性
- The protein encoded by the Matriptase gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion and metastasis.
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Suppression of Tumorigenicity 14 (ST14) ELISA Kit
ST14 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 2.5 h
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板类型
- Pre-coated
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实验流程
- The Matriptase (TADG-15) ELISA assay kit is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact Matriptase molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a biotin conjugated anti-Matriptase monoclonal antibody. After a second wash Streptavidin conjugated to HRP is added as a reporting agent. Excess streptavidin-HRP is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if Matriptase is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of Matriptase is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- ST14 (Suppression of Tumorigenicity 14 (ST14))
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别名
- Matriptase
抗原 See all ST14 ELISA试剂盒
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