IL-21 ELISA 试剂盒
Quick Overview for IL-21 ELISA 试剂盒 (ABIN2648760)
抗原
See all IL-21 (IL21) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human Interleukin 21 (IL-21) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 21 (IL-21) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 15 ng/ml
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产品特性
- Human Interleukin 21 (IL-21) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 21 (IL21) ELISA Kit
IL21 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitSmall Sample IL21 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 21 (IL21) ELISA KitIL21 适用: 小鼠 AA 25-146 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Interleukin 21 (IL21) ELISA KitIL21 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 21 (IL21) ELISA KitIL21 适用: Cow Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 21 (IL21) ELISA KitIL21 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- Human Interleukin 21 (IL-21) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-21 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-21 is added to the wells and binds to the combination of capture antibody- IL-21 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-21 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-21 standard dilutions and IL-21 sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL-21
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原 See all IL-21 (IL21) ELISA试剂盒
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