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IL-17 ELISA 试剂盒

This 人 IL-17 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL-17.
产品编号 ABIN2648755
发货至: 中国

Quick Overview for IL-17 ELISA 试剂盒 (ABIN2648755)

抗原

See all IL-17 (IL17) ELISA试剂盒
IL-17 (IL17) (Interleukin 17 (IL17))

适用

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检测方法

Colorimetric

应用范围

ELISA

样品类型

Serum, Plasma, Cell Culture Cells
  • 原理

    Human Interleukin 17 (IL-17) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 17 (IL-17) concentrations in cell culture supernates, serum, and plasma.

    Analytical Method

    Quantitative

    灵敏度

    7 pg/ml

    产品特性

    Human Interleukin 17 (IL-17) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    样本量

    100 μL

    实验时间

    3.5 h

    板类型

    Pre-coated

    实验流程

    Human Interleukin 17 (IL-17) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17 is added to the wells and binds to the combination of capture antibody-IL-17 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-17 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17 standard dilutions and IL-17 sample concentration determined.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all IL-17 (IL17) ELISA试剂盒

    IL-17 (IL17) (Interleukin 17 (IL17))

    别名

    IL-17
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