Vitamin B2 Whole Blood Microtiter Plate Assay
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北京 101111
Quick Overview for Vitamin B2 Whole Blood Microtiter Plate Assay (ABIN2648738)
抗原
See all Vitamin B2 试剂盒适用
应用范围
样品类型
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原理
- Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood.The test kit contains all required reagents, e.g.standard,medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.
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灵敏度
- 5 μg/L
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产品特性
- Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood.The test kit contains all required reagents, e.g.standard,medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.
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样本量
- 100 μL
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实验时间
- 2 d
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实验流程
- Pretreated EDTA whole blood samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus.The addition of Vitamin B2 in either standards [STD] or samples gives a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37 °C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 - 630nm (alternatively at 540 - 550nm) in an ELISA-reader and a standard curve is generated from the dilution series.The amount of Vitamin B2 is directly proportional to the turbidity.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Vitamin B2 (Vitamin B2 (Riboflavin))
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别名
- Vitamin B2
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物质类
- Chemical
抗原
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