IL-5 ELISA 试剂盒
Quick Overview for IL-5 ELISA 试剂盒 (ABIN2648700)
抗原
See all IL-5 (IL5) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human Interleukin 5 (IL-5) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 5 (IL-5) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 2 pg/ml
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产品特性
- Human Interleukin 5 (IL-5) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 5 (IL5) ELISA Kit
IL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 豚鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 山羊 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 绵羊 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 小鸡 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 5 (IL5) ELISA KitIL5 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- PRINCIPLE OF THE ASSAY Human Interleukin 5 (IL-5) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-5 is added to the wells and binds to the combination of capture antibody- IL-5 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-5 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-5 standard dilutions and IL-5 sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- IL-5
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原 See all IL-5 (IL5) ELISA试剂盒
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