ICAM1 ELISA 试剂盒
Quick Overview for ICAM1 ELISA 试剂盒 (ABIN2648681)
抗原
See all ICAM1 ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Human Intercellular Adhesion Molecule 1 (SICAM-1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Intercellular Adhesion Molecule 1 (sICAM-1) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 31 pg/ml
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产品特性
- Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
ICAM1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 人 AA 28-480 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 大鼠 AA 28-493 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 小鼠 AA 28-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sICAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sICAM-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for sICAM-1 is added to the wells and binds to the combination of capture antibody- sICAM-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of sICAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven sICAM-1 standard dilutions and sICAM-1 sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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别名
- sICAM-1
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物质类
- Viral Protein
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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