Insulin Autoantibody ELISA 试剂盒
The 人 Insulin Autoantibody ELISA Kit (ABIN2648679) is a Colorimetric ELISA Kit designed to quantify 人 Insulin Autoantibody.
产品编号 ABIN2648679
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Insulin Autoantibody ELISA 试剂盒 (ABIN2648679)
抗原
Insulin Autoantibody
适用
人
检测方法
Colorimetric
应用范围
ELISA
样品类型
Serum
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灵敏度
- 0.08 U/mL
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产品特性
- Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to an environmental agent. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80-90 % of the cells are lost. This process may take years to complete and may occur at any time in all ages.
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Insulin Autoantibody ELISA Kit
适用: 人 Colorimetric Indirect ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Autoantibody ELISA Kit适用: 人 Colorimetric Indirect ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 5 μL
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实验时间
- 1.5 h
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板类型
- Pre-coated
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实验流程
- In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Insulin Autoantibody
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别名
- IAA (Insulin Autoantibodies)
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物质类
- Antibody
抗原
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