Helicobacter Pylori Antigen ELISA 试剂盒
适用: Helicobacter pylori
Colorimetric
Fecal
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- 抗原
- Helicobacter Pylori Antigen
- 适用
- Helicobacter pylori
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 样品类型
- Fecal
- 灵敏度
- 0.165 ng/ml
- 产品特性
- H. pylori (previously known as Campylobacter pyloridis) is a type of bacteria that infects the stomach and is a common cause of peptic ulcers. H. pylori bacteria can be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori can also be spread through contaminated food or water.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 2 h
- 实验流程
- Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its wall. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of H. pylori is added for further immunoreactions. After an incubation period, the immunocomplex of "H. pylori Antibody - H. pylori Antigen - HRP-conjugated Anti-H. pylori Tracer Antibody" is formed if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Helicobacter Pylori Antigen
- 别名
- H Pylori Antigen
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