Homoarginine ELISA 试剂盒
This 人 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 .
Quick Overview for Homoarginine ELISA 试剂盒 (ABIN2648674)
抗原
Homoarginine
适用
人
检测方法
Colorimetric
应用范围
ELISA
样品类型
Serum, Plasma, Cell Culture Cells
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灵敏度
- 0.05 μmol/L
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产品特性
- Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) study have identified homoarginine as useful predictor of cardiovascular events and mortality.
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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实验时间
- 2.5 h
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板类型
- Pre-coated
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实验流程
- The competitive Homoarginine ELISA Assay Kit uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Homoarginine
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物质类
- Amino Acid
抗原
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