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GLP-1 ELISA 试剂盒

This 人, 小鼠 和 大鼠 GLP-1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 GLP-1.
产品编号 ABIN2648664
发货至: 中国

Quick Overview for GLP-1 ELISA 试剂盒 (ABIN2648664)

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GLP-1 (Glucagon-like peptide 1 (GLP-1))

抗原表位

total

适用

  • 13
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人, 小鼠, 大鼠

检测方法

Colorimetric

应用范围

ELISA

样品类型

Plasma
  • 灵敏度

    0.6 pmol/L

    产品特性

    The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    样本量

    100 μL

    实验流程

    Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36)/(9-36) - HRP-conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all GLP-1 ELISA试剂盒

    GLP-1 (Glucagon-like peptide 1 (GLP-1))

    别名

    GLP-1
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