GLP-1 ELISA 试剂盒
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Quick Overview for GLP-1 ELISA 试剂盒 (ABIN2648663)
抗原
See all GLP-1 ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 0.1 pM
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产品特性
- Mouse/Rat Active GLP-1 ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of bioactive glucagon-like peptide-1 (7-36) amide [GLP-1 (7-36)] level in rat and mouse plasma samples with only 20 μL of sample volume. E The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Glucagon-like peptide 1 (GLP-1) ELISA Kit
GLP-1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon-like peptide 1 (GLP-1) ELISA KitGLP-1 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Glucagon-like peptide 1 (GLP-1) ELISA KitSmall Sample GLP-1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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实验时间
- 1 d
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实验流程
- Mouse/Rat Active GLP-1 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma that usually have limited amount of sample available for analysis. The assay utilizes the two-site "sandwich" technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36) - HRP conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- GLP-1 (Glucagon-like peptide 1 (GLP-1))
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别名
- GLP-1
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