Dopamine ELISA 试剂盒
Quick Overview for Dopamine ELISA 试剂盒 (ABIN2648625)
抗原
See all Dopamine (DA) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- The Dopamine High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples.
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Analytical Method
- Quantitative
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特异性
- High Sensitive ELISA
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灵敏度
- 5.9 pg/mL
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产品特性
- The Dopamine High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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板类型
- Pre-coated
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实验流程
- The competitive Dopamine High Sensitive ELISA kit uses the microtitre plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Dopamine (DA)
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别名
- Dopamine
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物质类
- Chemical
抗原 See all Dopamine (DA) ELISA试剂盒
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