ACA-IgG ELISA 试剂盒
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Quick Overview for ACA-IgG ELISA 试剂盒 (ABIN2648608)
抗原
See all ACA-IgG products适用
检测方法
应用范围
样品类型
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灵敏度
- 0.08 μgEq/m
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产品特性
- The first study on the anti-phospholipid antibodies began in 1906 when Wasserman introduced a serological test for syphilis. In 1942 it was discovered that the active component is a phospholipid called Cardiolipina. In the 1950's it was observed that a large number of people appeared to be positive for syphilis tests but did not show any evidence of disease. Initially the phenomenon was classified as a series of false positive syphilis tests, before a more accurate analysis revealed, for this group of patients, a high prevalence of autoimmune disorders including SLE and Sjögrens syndrome.
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Anti-Cardiolipin Antibody IgG (ACA-IgG) ELISA Kit
ACA-IgG 适用: 小鼠 Colorimetric Indirect ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Anti-Cardiolipin Antibody IgG (ACA-IgG) ELISA KitACA-IgG 适用: 人 Colorimetric Indirect ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 10 μL
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实验时间
- 2 h
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实验流程
- Anti Cardiolipin IgG ELISA Assay Kit is based on the binding of antibodies on human serum or plasma directed against the antigenic complex between Cardiolipina and β2-Glycoprotein, this complex is coated on the microplate. In the first step the antibodies present in calibrators, controls or prediluted samples bind to this coated antigen. After a 60 minute incubation the microplate is washed with a wash buffer to remove the non-reactive serum or plasma components. Then an anti-human IgM horseradish peroxidase conjugated solution recognizes IgM class antibodies bound to the immobilized antigens. After a 60 minute incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Finally, achromogenic substrate solution containing TMB is dispensed into the wells. After a 15 minute incubation the color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgG antibodies present in the original sample. The concentration of IgG antibodies in the sample is calculated through a calibration curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- ACA-IgG (Anti-Cardiolipin Antibody IgG (ACA-IgG))
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别名
- ANTI CARDIOLIPIN IgG
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物质类
- Antibody
抗原 See all ACA-IgG products
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