Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody.
产品编号 ABIN2648603
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) ELISA 试剂盒 (ABIN2648603)
抗原
Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)
适用
人
检测方法
Colorimetric
应用范围
ELISA
样品类型
Serum, Plasma
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灵敏度
- 0.13 AU/mL
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产品特性
- Anti-neutrophilic-cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 10 μL
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实验时间
- 1.5 h
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实验流程
- Anti PR3 (c-ANCA) ELISA Assay Kit is a test is based on the binding of the antibodies in the sample with human neutrophil proteinase 3 coated into the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind to the inner surface of the wells. After a 60 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. In the second step an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate which is not specifically bound is washed away with the wash buffer.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)
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别名
- ANTI PR-3 (C-ANCA)
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物质类
- Antibody
抗原
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