Cortisol ELISA 试剂盒
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北京 101111
Quick Overview for Cortisol ELISA 试剂盒 (ABIN2648588)
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See all Cortisol ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 2.95 ng/ml
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产品特性
- Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress, it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors. These normal endogenous functions are the basis for the physiological consequences of chronic stress - prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.
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Cortisol ELISA Kit
适用: Various Species Colorimetric Sandwich ELISA Fecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA Kit适用: Various Species Colorimetric Sandwich ELISA 100 pg/mL - 3200 pg/mL Fecal, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA Kit适用: Various Species Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA Kit适用: 兔 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitHigh Sensitivity 适用: Various Species Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum, Urine
Cortisol ELISA Kit适用: 人 Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA Kit适用: 猴 Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 10 μL
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实验时间
- 1.5 h
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实验流程
- The Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Cortisol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the Cortisol concentration of in the sample.Cortisol concentration in the sample is calculated through a calibration curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Cortisol
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物质类
- Hormone
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