Hydroxypyridinium Crosslinks HPLC Assay
HPLC
Urine
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- 抗原
- Hydroxypyridinium Crosslinks
- 应用范围
- High Pressure Liquid Chromatography (HPLC)
- 原理
- Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not reutilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone. The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget's Disease, primary hyperparathyroidism and bone metastasis.
- 样品类型
- Urine
- 产品特性
- Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not reutilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone. The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget's Disease, primary hyperparathyroidism and bone metastasis.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 1 mL
- 实验时间
- 4.5 h
- 实验流程
- For the determination of the pyridinium crosslinks, the samples are hydrolyzed and extracted on solid phase extraction cartridges. The probe is centrifuged and 100 μL of the supernatant are injected into the HPLC system. The isocratic separation is via HPLC at 30 °C using a "reversed phase" column. One run lasts 15 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered plasma calibrator, the concentration is calculated via integration of the peak areas or heights.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Hydroxypyridinium Crosslinks
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