G-CSF ELISA 试剂盒
Quick Overview for G-CSF ELISA 试剂盒 (ABIN2648562)
抗原
See all G-CSF (CSF3) ELISA试剂盒适用
检测方法
应用范围
样品类型
-
-
原理
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Colony Stimulating Factor (G-CSF) concentrations in cell culture supernates, serum, and plasma.
-
Analytical Method
- Quantitative
-
灵敏度
- 4 pg/ml
-
产品特性
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
-
-
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA Kit
CSF3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 兔 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 人 AA 31-204 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 AA 31-208 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
样本量
- 100 μL
-
实验时间
- 3.5 h
-
板类型
- Pre-coated
-
实验流程
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for G-CSF is added to the wells and binds to the combination of capture antibody- G-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of G-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven G-CSF standard dilutions and G-CSF sample concentration determined.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
-
别名
- G-CSF
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原 See all G-CSF (CSF3) ELISA试剂盒
-
